Plasmid_Backbone
pSB1Tcs1

Part:BBa_K2725005:Experience

Designed by: Ben Astles   Group: iGEM18_Edinburgh_UG   (2018-10-11)


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Applications of BBa_K2725005

Using E. coli DH5-alpha transformed with BBa_K2725005, we produced growth curves at various concentrations of triclosan over a period of 22 hours (as seen in the figure below), which showed that it conferred triclosan resistance up to the limits of solubility. There is RFP in the BioBrick site, so BBa_K2725005 may be used for red/white screening. For liquid culture 4 micromolar triclosan should be used, while for plating 1 micromolar is sufficient for selection.

<img src="T--Edinburgh_UG--FabVgraph.jpeg" width=100% height=100%>

User Reviews

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