Plasmid_Backbone
pSB1Tcs1
Part:BBa_K2725005:Experience
Designed by: Ben Astles Group: iGEM18_Edinburgh_UG (2018-10-11)
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Applications of BBa_K2725005
Using E. coli DH5-alpha transformed with BBa_K2725005, we produced growth curves at various concentrations of triclosan over a period of 22 hours (as seen in the figure below), which showed that it conferred triclosan resistance up to the limits of solubility. There is RFP in the BioBrick site, so BBa_K2725005 may be used for red/white screening. For liquid culture 4 micromolar triclosan should be used, while for plating 1 micromolar is sufficient for selection.
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User Reviews
UNIQ1bc05d39fff8caf3-partinfo-00000000-QINU UNIQ1bc05d39fff8caf3-partinfo-00000001-QINU